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Human RAD54 inhibitor screen: summary of 158 compounds identified as 'hits'

Citation

Ehmsen, Kirk et al. (2018), Human RAD54 inhibitor screen: summary of 158 compounds identified as 'hits', UC San Francisco Dash, Dataset, https://doi.org/10.7272/Q68G8HW8

Abstract

This dataset, Human RAD54 inhibitor screen: summary of 158 compounds identified as 'hits', accompanies the manuscript, Small molecule inhibitors of a human recombination-associated ATPase, RAD54.  158 compounds identified as inhibitory to the ATPase activity of purified human RAD54 on dsDNA (pUC19) in a primary malachite green phosphate detection assay are catalogued in an Excel document, with annotation describing follow-up in secondary assays (including IC50 values, DNA dependence of ATPase inhibition & class assignments, TopoI inhibition, structural similarity to known DNA intercalators, SF50 (toxicity to HEK293), combination index (CI) with mitomycin C (MMC) or cisplatin (CsPt), reversibility/irreversibility of RAD54 inhibition). 

Methods

Please refer to Materials and Methods of the associated manuscript, Small molecule inhibitors of the human recombination-associated ATPase, RAD54.  Data in fields for secondary assays were manually curated.

Usage Notes

Please refer to the associated manuscript, Small molecule inhibitors of the human recombination-associated ATPase, RAD54, for details related to derivation and curation of this dataset.

Funding

United States Department of Defense (DoD), Award: BC083684

QB3-Malaysia Program,